Uniform Serial Sectioning for Transmission Electron Microscopy

Uniform serial sectioning for transmission electron microscopy.

Kristen M. Harris,1,2,3 Elizabeth Perry,2 Jennifer Bourne,1,2 Marcia Feinberg,3 Linnaea Ostroff,3 and Jamie Hurlburt2,3 1Section of Neurobiology and Center for Learning and Memory, University of Texas at Austin, Austin, Texas 78712, 2Department of Neurology, Synapses, and Cognitive Neuroscience Center, Medical College of Georgia, Augusta, Georgia 30912, 3Program in Neuroscience and Biology Depa...

Sectioning of tissue for electron microscopy.

Improved Serial Sectioning Techniques for Correlative Light-Electron Microscopy Mapping of Human Langerhans Islets

Combined analysis of immunostaining for various biological molecules coupled with investigations of ultrastructural features of individual cells is a powerful approach for studies of cellular functions in normal and pathological conditions. However, weak antigenicity of tissues fixed by conventional methods poses a problem for immunoassays. This study introduces a method of correlative light an...

A model based reconstruction technique for depth sectioning with scanning transmission electron microscopy

Depth sectioning in high angular annular dark field scanning transmission electron microscopy is considered a candidate for three-dimensional characterization on the atomic scale. However at present the depth resolution is still far from the atomic level, due to strong limitations in the opening angle of the beam. In this paper we introduce a new, parameter based tomographic reconstruction algo...

Non-uniform Illumination Correction in Transmission Electron Microscopy

Transmission electron microscopy (TEM) provides resolutions on the order of a nanometer. Hence, it is a critical imaging modality for biomedical analysis at the sub-cellular level. One of the problems associated with TEM images is variations in brightness due to electron imaging defects or non-uniform support films and specimen staining. These variations render image processing operations such ...